Intensity-Based Camera Setup for Refractometric and Biomolecular Sensing with a Photonic Crystal Microfluidic Chip

Label-free sensing is a promising approach for point-of-care testing devices. Among optical transducers, photonic crystal slabs (PCSs) have positioned themselves as an inexpensive yet versatile platform label-free biosensing. A spectral resonance shift observed upon biomolecular binding to the functionalized surface. Commonly, PCS read out by spectrometer. Alternatively, may be translated into intensity change tailoring system response. Intensity-based camera setups (IBCS) are of interest they mitigate need postprocessing, enable spatial sampling, and moderate hardware requirements. However, exhibit modest performance compared with spectrometric approaches. Here, we show increase sensitivity limit detection (LOD) IBCS employing sharp-edged cut-off filter optimize We report LOD from (7.1 ± 1.3) × 10−4 RIU (3.2 0.7) 10−5 RIU. discuss influence region (ROI) size on achievable LOD. fabricated biochip combining microfluidic demonstrated autonomous transport. analyzed via refractive index steps biosensing ability diluted glutathione S-transferase (GST) antibodies (1:250). In addition, illustrate speed demonstrate advantage additional information detecting streptavidin (2.9 µg/mL). Finally, present immunoglobulin G (IgG) whole blood possible basis